LOCUS       sequence-71552-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  TITLE     Jorgensen unpublished mosSCI reagents
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCFJ210
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             354..659
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     538..557
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ggcggggcgt aatctagagg atccggctta ctaaaagcca gataacagta tgcgtatttg
       61 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
      121 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
      181 tcaaggcata tatgatgtca atatctccgg ttcggtaagc acaaccatgc agaatgaagc
      241 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
      301 ccggtttatt gaaatgaacg gctcttttgc cgacgagaac aggggctggt gaaatgcagt
      361 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
      421 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
      481 cagataaagt cccccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
      541 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
      601 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
      661 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
      721 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
      781 atatcatttt acgtttctcg ttcaactttt ctatacaaag tt
//
