LOCUS       sequence-71554-e         547 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For knock-in donor vector by CRISPR/CAS system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R
  TITLE     One-Step Generation of Mice Carrying Reporter and Conditional 
            Alleles by CRISPR/Cas-Mediated Genome Engineering.
  JOURNAL   Cell. 2013 Aug 27. pii: S0092-8674(13)01016-7. doi: 
            10.1016/j.cell.2013.08.022.
  PUBMED    23992847
REFERENCE   2  (bases 1 to 547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Aug 27. pii: S0092-8674(13)01016-7. doi: 
            10.1016/j.cell.2013.08.022."
COMMENT     Sequence Label: Oct4-IRES-eGFP-PGK-Neo
FEATURES             Location/Qualifiers
     source          1..547
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..18
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2..18
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      406..415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctctagaact agtggatccc ccgggctgca ggaattctcc atagcacagg ggctcgtgga
       61 aaccaagcat ggagttgggc aggagaagac tatgagtcaa ggcacttgct gccaagccta
      121 gcgacctgga cttgacccac aggacccaca ccatggaggg aaccaacttt tttcctcaca
      181 ttgtgacaat cttcacacat aaatatgcac atacttactg tgtccaggcc agcttggtct
      241 tcatgagata ctggggggag gggaggggag ggaggcagag atctggtagg gctgacattc
      301 agcagcaaaa atgctttaag acttggcttt gggggctgga gagatggctc agcggttaag
      361 agcactgact gctctgccag aggtccagag ttcaattccc atcaaccatg gtggctcaca
      421 accatctgta atgggatcca ttgccctctt ctggtgtgtc tgaagacagc tcaaatacat
      481 aaaataaata actttttttt tttctaaagg agctgcttgg ctcctcctct cttttttttt
      541 tttaatt
//
