LOCUS       sequence-71570-e        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  TITLE     Series-14: N-terminal Tag E.coli T7 Expression Vectors with Biobrick
            POLYPROMOTER Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET StrepII TEV expression vector with BioBric...
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      61..88
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        114..132
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             149..171
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             187..210
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             217..237
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      279..325
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctgttgttt gtcggtgagt ccttccagtc tgggcgaaag cgcggtcgat caaattaagc
       61 agaaggccat cctgacggat ggcctttttg cgtttctatc gatcccgcga aattaatacg
      121 actcactata ggcccctcta gaaataattt tgtttaactt taagaaggag atataccatg
      181 gctagctgga gccacccgca gttcgaaaaa ggcgccgaaa acctgtactt ccaatccaat
      241 attggaagtg gataactggc tgctgccacc gctgagcata gcataacccc ttggggcctc
      301 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatcaagtc ccgaagtagc
      361 tgtcagactg ctggcgaccg ccgcaactag tggcgtcatg cgtgccgtat ggtctttcgc
      421 ctgtagacgc cctacaagcc gtactaaagt ggaaagaccc gcaaaaggta tcgcgatcgc
      481 cctgcaggga actatatccg actagtagcc cgcctaatga gcgggctttt ttttaattcc
      541 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
      601 tgataaatgc ttcgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      661 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct cgtgaaagta
      721 aaagacgcag aggaccaatt gggggcacga gtgggataca tagaactgga cttgaatagc
      781 ggtaaaatcc ttgagagttt tcgccctgaa gagcgttttc caatgatgag cactttcaaa
      841 gttctgctat gtggagcagt attatcccgt gtagatgcgg ggcaagagca actcggacga
      901 cgaatacact attcgcagaa tgacttggtt gaatactccc cagtgacaga aaagcacctt
      961 acggacggaa tgacggtaag agaattatgt agtgccgcca taacgatgag tgataacact
     1021 gcggcgaact tacttctgac aaccatcggt ggaccgaagg aattaaccgc ttttt
//
