LOCUS       sequence-71576-e        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  TITLE     8-series E. coli arabinose expression vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBAD His6 MBP TEV LIC transfer vector (8CT)
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             105..127
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             147..164
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt ttttgggcta
       61 ttaattaagc ggccgccctg caggactcga gttctagaaa taattttgtt taactttaag
      121 aaggagatat acatatgaaa tcttctcacc atcaccatca ccatggttct tctatgaaaa
      181 tcgaagaagg taaactggta atctggatta acggcgataa aggctataac ggtctcgctg
      241 aagtcggtaa gaaattcgag aaagataccg gaattaaagt caccgttgag catccggata
      301 aactggaaga gaaattccca caggttgcgg caactggcga tggccctgac attatcttct
      361 gggcacacga ccgctttggt ggctacgctc aatctggcct gttggctgaa atcaccccgg
      421 acaaagcgtt ccaggacaag ctgtatccgt ttacctggga tgccgtacgt tacaacggca
      481 agctgattgc ttacccgatc gctgttgaag cgttatcgct gatttataac aaagatctgc
      541 tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct ggataaagaa ctgaaagcga
      601 aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg ccgctgattg
      661 ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt aaagacgtgg
      721 gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg attaaaaaca
      781 aacacatgaa tgcagacacc gattactcca tcgcagaagc tgcctttaat aaaggcgaaa
      841 cagcgatgac catcaacggc ccgtgggcat ggtccaacat cgacaccagc aaagtgaatt
      901 atggtgtaac ggtactgccg accttcaagg gtcaaccatc caaaccgttc gttggcgtgc
      961 tgagcgcagg tattaacgcc gccagtccga acaaagagct ggcaaaagag ttcctcgaaa
     1021 actatctgct gactgatgaa ggtctggaag cggttaataa agacaaaccg ctgggtgccg
     1081 ta
//
