LOCUS       sequence-71582-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral plasmid for co-expressing siRNA-resistant, FKBP-tagged 
            AP-1 gamma subunit and G418 resistance gene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Robinson MS, Sahlender DA, Foster SD
  TITLE     Rapid inactivation of proteins by rapamycin-induced rerouting to 
            mitochondria.
  JOURNAL   Dev Cell. 2010 Feb 16;18(2):324-31. doi: 
            10.1016/j.devcel.2009.12.015.
  PUBMED    20159602
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2009.12"; journalName: "Dev Cell"; date: 
            "2010-02-16- 16"; volume: "18"; issue: "2"; pages: "324-31"
COMMENT     Sequence Label: pLXIN-Ap1g1-FKBP
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             135..935
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(198..217)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     808..827
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 agtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta
       61 gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa
      121 aacacgatga taatatggga tcggccattg aacaagatgg attgcacgca ggttctccgg
      181 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
      241 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
      301 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
      361 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
      421 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
      481 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
      541 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
      601 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
      661 ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt gacccatggc gatgcctgct
      721 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
      781 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
      841 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
      901 gcatcgcctt ctatcgcctt cttgacgagt tcttctg
//
