LOCUS       sequence-71585-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E-cadherin EC12 WT with C9A mutation and avi tag in 
            E.Coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Ritterson RS, Kuchenbecker KM, Michalik M, Kortemme T
  TITLE     Design of a Photoswitchable Cadherin.
  JOURNAL   J Am Chem Soc. 2013 Aug 7.
  PUBMED    23923816
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2013 Aug 7."
COMMENT     Sequence Label: pET22b-EC12WTC9AAvi
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    62..86
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..123
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             134..151
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             833..877
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             887..904
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
       61 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacat atgcaccacc accaccacca cgaaaacctg tacttccagg actgggtcat
      181 ccctcccatc agcgcccccg aaaatgaaaa gggcgaattt ccaaagaacc tggttcagat
      241 caaatccaac agggacaaag aaacaaaggt tttctacagc atcaccggcc aaggagctga
      301 caaacccccc gttggcgttt tcatcattga gagggagaca ggctggctga aagtgacaca
      361 gcctctggat agagaagcca ttgccaagta catcctctat tctcatgccg tgtcgtcaaa
      421 tggggaagcg gtggaggatc ccatggagat agtgatcaca gtgacagatc agaatgacaa
      481 caggccagag tttacccagc cggtctttga gggattcgtt gcagaaggcg ctgttccagg
      541 aacctccgtg atgaaggtct cagccaccga tgcagacgat gacgtcaaca cctacaacgc
      601 tgccatcgcc tacaccatcg tcagccagga tcctgagctg cctcacaaaa acatgttcac
      661 tgtcaatagg gacaccgggg tcatcagtgt gctcacctct gggctggacc gagagagtta
      721 ccctacatac actctggtgg ttcaggctgc tgaccttcaa ggtgaaggct tgagcacaac
      781 agccaaggct gtgatcactg tcaaggatat taatgacaac gctcctggtg gcggtctgaa
      841 cgacatcttc gaggctcaga aaatcgaatg gcacgaatga ctcgagcacc accaccacca
      901 ccactgagat ccggctgcta acaaagcccg aa
//
