LOCUS       sequence-71586-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli genomic integration at arsB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Sabri S, Steen JA, Bongers M, Nielsen LK, Vickers CE
  TITLE     Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA
            sequences, including whole metabolic pathways, onto the Escherichia 
            coli chromosome at well-characterised loci.
  JOURNAL   Microb Cell Fact. 2013 Jun 24;12(1):60.
  PUBMED    23799955
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-06-24"; volume: "12(1)"; pages: "60"
COMMENT     Sequence Label: pKIKOarsBCm
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    47..94
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tactgcgatg agtggcaggg cggggcgtaa ggcgcgccat ttaaatgaag ttcctattcc
       61 gaagttccta ttctctagaa agtataggaa cttcgaagca gctccagcct acgattaaaa
      121 aaaaccccgc cgaagcgggg ttttttttgt ttatcgccgc gattgttgcc acgctggtga
      181 tgttacatct ctattttcgc aaagatattc cgcagaacta cgatatggcg ctgctgaaat
      241 ctcccgcaga agcgatcaaa gatcctgcta cgttcaaaac tggctgggtt gttttactgc
      301 ttctgctggt gggatttttc gtcctggaac cgctcggcag tccggtgagc gccattgcag
      361 ctgtgggcgc gctgatatta tttgtcgtcg ctaaacgcgg tcatgcgatt aatacgggta
      421 aagtcctgcg cggtgccccc tggcagattg tcatcttctc gctcggcatg tatctggtgg
      481 tttatggcct gcgcaatgcc ggattaacgg aatatctttc tggcgtactc aacgtgctgg
      541 cggataacgg cctgtgggcc gcgacgctcg gcaccggatt cctcaccgcc ttcctctctt
      601 ctattatgaa caatatgccg acggtactgg ttggcgcgtt gtccattgat ggcagcacgg
      661 catctggcgt tatcaaagaa gcgatggttt atgccaatgt gattggctgc gatttgggac
      721 gtttctggat gcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      781 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      841 ataatattga
//
