LOCUS       sequence-71588-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E-cadherin EC12 with C9A, K129C/D138C mutations and avi 
            tag in E.Coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Ritterson RS, Kuchenbecker KM, Michalik M, Kortemme T
  TITLE     Design of a Photoswitchable Cadherin.
  JOURNAL   J Am Chem Soc. 2013 Aug 7.
  PUBMED    23923816
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2013 Aug 7."
COMMENT     Sequence Label: pET22b-EC12-129/138Avi
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        44..62
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    63..87
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             102..124
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             135..152
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             834..878
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             888..905
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aattaatacg actcactata
       61 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
      121 gagatataca tatgcaccac caccaccacc acgaaaacct gtacttccag gactgggtca
      181 tccctcccat cagcgccccc gaaaatgaaa agggcgaatt tccaaagaac ctggttcaga
      241 tcaaatccaa cagggacaaa gaaacaaagg ttttctacag catcaccggc caaggagctg
      301 acaaaccccc cgttggcgtt ttcatcattg agagggagac aggctggctg aaagtgacac
      361 agcctctgga tagagaagcc attgccaagt acatcctcta ttctcatgcc gtgtcatcaa
      421 atggggaagc ggtggaggat cccatggaga tagtgatcac agtgacagat cagaatgaca
      481 acaggccaga gtttacccag ccggtctttg agggattcgt tgcagaaggc gctgttccag
      541 gaacctccgt gatgtgcgtc tcagccaccg atgcagacga ttgcgtcaac acctacaacg
      601 ctgccatcgc ctacaccatc gtcagccagg atcctgagct gcctcacaaa aacatgttca
      661 ctgtcaatag ggacaccggg gtcatcagtg tgctcacctc tgggctggac cgagagagtt
      721 accctacata cactctggtg gttcaggctg ctgaccttca aggtgaaggc ttgagcacaa
      781 cagccaaggc tgtgatcact gtcaaggata ttaatgacaa cgctcctggt ggcggtctga
      841 acgacatctt cgaggctcag aaaatcgaat ggcacgaatg actcgagcac caccaccacc
      901 accactgaga tccggctgct aacaaagccc gaaaggaagc tga
//
