LOCUS       sequence-71589-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli genomic integration at lacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Sabri S, Steen JA, Bongers M, Nielsen LK, Vickers CE
  TITLE     Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA
            sequences, including whole metabolic pathways, onto the Escherichia 
            coli chromosome at well-characterised loci.
  JOURNAL   Microb Cell Fact. 2013 Jun 24;12(1):60.
  PUBMED    23799955
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-06-24"; volume: "12(1)"; pages: "60"
COMMENT     Sequence Label: pKIKOlacZCm
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    45..92
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        701..805
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcgatgag tggcagggcg gggcgtaagg cgcgccattt aaatgaagtt cctattccga
       61 agttcctatt ctctagaaag tataggaact tcgaagcagc tccagcctac gattaaaaaa
      121 aaccccgccg aagcggggtt ttttttgttt atcctgctga tgaagcagaa caactttaac
      181 gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac
      241 ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
      301 ctgaccgatg atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag
      361 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc
      421 gctaatcacg acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag
      481 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
      541 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg
      601 ctacctggag agatgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt
      661 cttgtttctg gatgcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      721 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      781 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      841 cttttttgcg
//
