LOCUS       sequence-71592-e         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E-cadherin EC12 WT with C9A mutation and avi tag in 
            E.Coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Ritterson RS, Kuchenbecker KM, Michalik M, Kortemme T
  TITLE     Design of a Photoswitchable Cadherin.
  JOURNAL   J Am Chem Soc. 2013 Aug 7.
  PUBMED    23923816
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2013 Aug 7."
COMMENT     Sequence Label: pET22b-EC12WTC9AAvi
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..29)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(39..83)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(765..782)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             793..815
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    830..854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(854..873)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(855..873)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gccggatctc agtggtggtg gtggtggtgc tcgagtcatt cgtgccattc gattttctga
       61 gcctcgaaga tgtcgttcag accgccacca ggagcgttgt cattaatatc cttgacagtg
      121 atcacagcct tggctgttgt gctcaagcct tcaccttgaa ggtcagcagc ctgaaccacc
      181 agagtgtatg tagggtaact ctctcggtcc agcccagagg tgagcacact gatgaccccg
      241 gtgtccctat tgacagtgaa catgtttttg tgaggcagct caggatcctg gctgacgatg
      301 gtgtaggcga tggcagcgtt gtaggtgttg acgtcatcgt ctgcatcggt ggctgagacc
      361 ttcatcacgg aggttcctgg aacagcgcct tctgcaacga atccctcaaa gaccggctgg
      421 gtaaactctg gcctgttgtc attctgatct gtcactgtga tcactatctc catgggatcc
      481 tccaccgctt ccccatttga cgacacggca tgagaataga ggatgtactt ggcaatggct
      541 tctctatcca gaggctgtgt cactttcagc cagcctgtct ccctctcaat gatgaaaacg
      601 ccaacggggg gtttgtcagc tccttggccg gtgatgctgt agaaaacctt tgtttctttg
      661 tccctgttgg atttgatctg aaccaggttc tttggaaatt cgcccttttc attttcgggg
      721 gcgctgatgg gagggatgac ccagtcctgg aagtacaggt tttcgtggtg gtggtggtgg
      781 tgcatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
      841 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct
      901 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
      961 tatcgccgac atcac
//
