LOCUS       sequence-71593-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E-cadherin EC12 with C9A, K129C/D138C mutations in 
            E.Coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Ritterson RS, Kuchenbecker KM, Michalik M, Kortemme T
  TITLE     Design of a Photoswitchable Cadherin.
  JOURNAL   J Am Chem Soc. 2013 Aug 7.
  PUBMED    23923816
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2013 Aug 7."
COMMENT     Sequence Label: pET22b-EC12-129/138
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(717..734)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             745..767
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    782..806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(806..825)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(807..825)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(909..928)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtc aaggagcgtt gtcattaata
       61 tccttgacag tgatcacagc cttggctgtt gtgctcaagc cttcaccttg aaggtcagca
      121 gcctgaacca ccagagtgta tgtagggtaa ctctctcggt ccagcccaga ggtgagcaca
      181 ctgatgaccc cggtgtccct attgacagtg aacatgtttt tgtgaggcag ctcaggatcc
      241 tggctgacga tggtgtaggc gatggcagcg ttgtaggtgt tgacgcaatc gtctgcatcg
      301 gtggctgaga cgcacatcac ggaggttcct ggaacagcgc cttctgcaac gaatccctca
      361 aagaccggct gggtaaactc tggcctgttg tcattctgat ctgtcactgt gatcactatc
      421 tccatgggat cctccaccgc ttccccattt gatgacacgg catgagaata gaggatgtac
      481 ttggcaatgg cttctctatc cagaggctgt gtcactttca gccagcctgt ctccctctca
      541 atgatgaaaa cgccaacggg gggtttgtca gctccttggc cggtgatgct gtagaaaacc
      601 tttgtttctt tgtccctgtt ggatttgatc tgaaccaggt tctttggaaa ttcgcccttt
      661 tcattttcgg gggcgctgat gggagggatg acccagtcct ggaagtacag gttttcgtgg
      721 tggtggtggt ggtgcatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag
      781 gggaattgtt atccgctcac aattccccta tagtgagtcg tattaatttc gcgggatcga
      841 gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
      901 tgctggcgcc tatatcgccg acatcaccga
//
