LOCUS       sequence-71594-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E-cadherin EC12 with C9A, K129C/D138C mutations and avi 
            tag in E.Coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Ritterson RS, Kuchenbecker KM, Michalik M, Kortemme T
  TITLE     Design of a Photoswitchable Cadherin.
  JOURNAL   J Am Chem Soc. 2013 Aug 7.
  PUBMED    23923816
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2013 Aug 7."
COMMENT     Sequence Label: pET22b-EC12-129/138Avi
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(42..86)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(768..785)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             796..818
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    833..857
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtc attcgtgcca ttcgattttc
       61 tgagcctcga agatgtcgtt cagaccgcca ccaggagcgt tgtcattaat atccttgaca
      121 gtgatcacag ccttggctgt tgtgctcaag ccttcacctt gaaggtcagc agcctgaacc
      181 accagagtgt atgtagggta actctctcgg tccagcccag aggtgagcac actgatgacc
      241 ccggtgtccc tattgacagt gaacatgttt ttgtgaggca gctcaggatc ctggctgacg
      301 atggtgtagg cgatggcagc gttgtaggtg ttgacgcaat cgtctgcatc ggtggctgag
      361 acgcacatca cggaggttcc tggaacagcg ccttctgcaa cgaatccctc aaagaccggc
      421 tgggtaaact ctggcctgtt gtcattctga tctgtcactg tgatcactat ctccatggga
      481 tcctccaccg cttccccatt tgatgacacg gcatgagaat agaggatgta cttggcaatg
      541 gcttctctat ccagaggctg tgtcactttc agccagcctg tctccctctc aatgatgaaa
      601 acgccaacgg ggggtttgtc agctccttgg ccggtgatgc tgtagaaaac ctttgtttct
      661 ttgtccctgt tggatttgat ctgaaccagg ttctttggaa attcgccctt ttcattttcg
      721 ggggcgctga tgggagggat gacccagtcc tggaagtaca ggttttcgtg gtggtggtgg
      781 tggtgcatat gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt
      841 tatccgctca caattcccct ata
//
