LOCUS       sequence-71595-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli genomic integration rbsAR locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Sabri S, Steen JA, Bongers M, Nielsen LK, Vickers CE
  TITLE     Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA
            sequences, including whole metabolic pathways, onto the Escherichia 
            coli chromosome at well-characterised loci.
  JOURNAL   Microb Cell Fact. 2013 Jun 24;12(1):60.
  PUBMED    23799955
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-06-24"; volume: "12(1)"; pages: "60"
COMMENT     Sequence Label: pKIKOrbsARCm
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..85
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gagtggcagg gcggggcgta aggcgcgcca tttaaatgaa gttcctattc cgaagttcct
       61 attctctaga aagtatagga acttcgaagc agctccagcc tacgattaaa aaaaaccccg
      121 ccgaagcggg gttttttttg tttcgattgt ctcgacgtta agcccgaact ttttcaccat
      181 caataactta ttcaatattc tccagcaaac ctcggtgaac gccattatgg cggtcgggat
      241 gacgctggtg atcctgacgt cgggcatcga cttgtctgta ggttctctgt tggcgctaac
      301 cggcgcagtt gctgcatcta tcgtcggcat tgaagtcaat gcgctggtgg ctgttactgt
      361 gcaccgaaac gcatcaacct tcgcgtgaaa tcatgcaacg ttatccgaca gtgcctactg
      421 tgatgatgga ctgggctccg ttcgatggcg acagcgatct tattcaggat aactcgttgc
      481 tgggcggaga cttagcaacg caatatctga tcgataaagg tcatacccgt atcgcctgta
      541 ttaccggtcc gctggataaa actccggcgc gcctgcggtt ggaaggttat cgggcggcga
      601 tgaaacgtgc gggtctcaac attcctgatg gctatgaagt cactggtgat tttgaattta
      661 acggcgggtt tgacgccatg cgccaactgt tatcacatcc gctgcgtcct caggccgtct
      721 ttaccggaaa tgacgctatg gctgttggcg tttaccaggc gttatatcag gcagagttac
      781 aggttccgca ggatatcgcg gtgattggct atgacgatat cgaactggca agctttatga
      841 cgccaccatt aaccactatc cacca
//
