LOCUS       sequence-71597-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and HXT7 promoter controlled expression cassettes with 
            phleomycin resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G4-Ph
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(173..196)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aaatcataaa tcataagaaa ttcgcttatt tagaagtgtc aacaacgtat ctaccaacga
       61 tttgaccctt ttccatcttt tcgtaaattt ctggcaaggt agacaagccg acaaccttga
      121 ttggagactt gaccaaacct ctggcgaaga attgttaatt aagagctcag atcttatcgt
      181 cgtcatcctt gtaatccatc gatactagtt ttttgattaa aattaaaaaa actttttgtt
      241 tttgtgttta ttctttgttc ttagaaaaga caagttgagc ttgtttgttc ttgatgtttt
      301 attattttac aatagctgca aatgaagaat agattcgaac attgtgaagt attggcatat
      361 atcgtctcta tttatacttt ttttttttca gttctagtat attttgtatt ttcctccttt
      421 tcattctttc agttgccaat aagttacagg ggatctcgaa agatggtggg gatttttcct
      481 tgaaagacga ctttttgcca tctaattttt ccttgttgcc tctgaaaatt atccagcaga
      541 agcaaatgta aaagatgaac ctcagaagaa cacgcagggg cccgaaattg ttcctacgag
      601 aagtagtggg tcataaaaag tttattccct ggaaaaaaaa ttttgcgttg cctttctgga
      661 gaattttttc gaattagcgt gctgccactg catgcatttc tgagaagtgt gggcattctt
      721 ccaccagttg ttcctcctaa aaaaaaaaag atttcctacc ccgcacaaat tcctgcatac
      781 ccctcatttc cacggggccg gccgcacaca ccatagctt
//
