LOCUS       sequence-71601-e        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  TITLE     8-series E. coli arabinose expression vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBAD empty polycistronic destination vector (8D)
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(180..196)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(253..537)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(311..330)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
ORIGIN
        1 aatcttctct catccgccaa aacagccagg ccggccagta ctttaattaa cgtctatcag
       61 ggcgatggca ccaggtgcgc gagcggccgc gactgggaaa accctggcgg gcgcgccgat
      121 atccctgcag gatgtgaaat tgttatccgc tgcgatcgcc ccgggactcg agtgaattca
      181 ctggccgtcg ttttacagga tcttgtacag ggcccggatc cgaggccttc tagatagccc
      241 aaaaaaacgg gtatggagaa acagtagaga gttgcgataa aaagcgtcag gtaagatccg
      301 ctaatcttat ggataaaaat gctatggcat agcaaagtgt gacgccgtgc aaataatcaa
      361 tgtggacttt tctgccgtga ttatagacac ttttgttacg cgtttttgtc atggctttgg
      421 tcccgctttg ttacagaatg cttttaataa gcggggttac cggtttggtt agcgagaaga
      481 gccagtaaaa gacgcagtga cggcaatgtc tgatgcaata tggacaattg gtttcttctc
      541 tgaatggcgg gagtatgaaa agtatggctg aagcgcaaaa tgatcccctg ctgccgggat
      601 actcgtttaa tgcccatctg gtggcgggtt taacgccgat tgaggccaac ggttatctcg
      661 atttttttat cgaccgaccg ctgggaatga aaggttatat tctcaatctc accattcgcg
      721 gtcagggggt ggtgaaaaat cagggacgag aatttgtttg ccgaccgggt gatattttgc
      781 tgttcccgcc aggagagatt catcactacg gtcgtcatcc ggaggctcgc gaatggtatc
      841 accagtgggt ttactttcgt ccgcgcgcct actggcatga atggcttaac tggccgtcaa
      901 tctttgccaa tacggggttc tttcgcccgg atgaagcgca ccagccgcat ttcagcgacc
      961 tgtttgggca aatcattaac gccgggcaag gggaagggcg ctattcggag ctgctggcga
     1021 taaatctgct tgagcaattg ttgctgcggc gcatggaagc gattaacgag tcgctccatc
     1081 caccgatgga ta
//
