LOCUS       sequence-71603-e        1113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac His6 MBP TEV cloning vector with BioB...
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(249..269)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(327..350)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatcc tctagtactt ctcgacaagc ttggtaccgc atgcctcgag tgcggcccct
      181 aggagatcat gaggccgcaa gcttgtcgac ggcgctcgaa ttcggatccg ttatccactt
      241 ccaatattgg attggaagta caggttttcc tcgatcccga ggttgttgtt attgttattg
      301 ttgttgttgt tcgagctcga attagtctgc gcgtctttca gggcttcatc gacagtctga
      361 cgaccgctgg cggcgttgat caccgcagta cgcacggcat accagaaagc ggacatctgc
      421 gggatgttcg gcatgatttc acctttctgg gcgttttcca tagtggcggc aatacgtgga
      481 tctttcgcca actcttcctc gtaagacttc agcgctacgg cacccagcgg tttgtcttta
      541 ttaaccgctt ccagaccttc atcagtcagc agatagtttt cgaggaactc ttttgccagc
      601 tctttgttcg gactggcggc gttaatacct gcgctcagca cgccaacgaa cggtttggat
      661 ggttgaccct tgaaggtcgg cagtaccgtt acaccataat tcactttgct ggtgtcgatg
      721 ttggaccatg cccacgggcc gttgatggtc atcgctgttt cgcctttatt aaaggcagct
      781 tctgcgatgg agtaatcggt gtctgcattc atgtgtttgt ttttaatcag gtcaaccagg
      841 aaggtcagac ccgctttcgc gccagcgtta tccacgccca cgtctttaat gtcgtacttg
      901 ccgttttcat acttgaacgc ataacccccg tcagcagcaa tcagcggcca ggtgaagtac
      961 ggttcttgca ggttgaacat cagcgcgctc ttacctttcg ctttcagttc tttatccagc
     1021 gccgggatct cttcccaggt ttttggcggg ttcggcagca gatctttgtt ataaatcagc
     1081 gataacgctt caacagcgat cgggtaagca atc
//
