LOCUS       sequence-71604-e        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac His6 MBP TEV cloning vector with BioB...
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             101..118
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgttttcgta acagttttgt aataaaaaaa cctataacta ttccggatta ttcataccgt
       61 cccaccatcg ggcgcggatc ccggaccgat gggttcttct caccatcacc atcaccatgg
      121 ttcttctatg aaaatcgaag aaggtaaact ggtaatctgg attaacggcg ataaaggcta
      181 taacggtctc gctgaagtcg gtaagaaatt cgagaaagat accggaatta aagtcaccgt
      241 tgagcatccg gataaactgg aagagaaatt cccacaggtt gcggcaactg gcgatggccc
      301 tgacattatc ttctgggcac acgaccgctt tggtggctac gctcaatctg gcctgttggc
      361 tgaaatcacc ccggacaaag cgttccagga caagctgtat ccgtttacct gggatgccgt
      421 acgttacaac ggcaagctga ttgcttaccc gatcgctgtt gaagcgttat cgctgattta
      481 taacaaagat ctgctgccga acccgccaaa aacctgggaa gagatcccgg cgctggataa
      541 agaactgaaa gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac cgtacttcac
      601 ctggccgctg attgctgctg acgggggtta tgcgttcaag tatgaaaacg gcaagtacga
      661 cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct tcctggttga
      721 cctgattaaa aacaaacaca tgaatgcaga caccgattac tccatcgcag aagctgcctt
      781 taataaaggc gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca acatcgacac
      841 cagcaaagtg aattatggtg taacggtact gccgaccttc aagggtcaac catccaaacc
      901 gttcgttggc gtgctgagcg caggtattaa cgccgccagt ccgaacaaag agctggcaaa
      961 agagttcctc gaaaactatc tgctgactga tgaaggtctg gaagcggtta ataaagacaa
     1021 accgctgggt gccgtagcgc tgaagtctta c
//
