LOCUS       sequence-71606-e        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac StrepII msfGFP TEV cloning vector wit...
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(268..288)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(join(298..1008,1009..1011))
                     /codon_start=1
                     /product="enhanced GFP (EGFP) derivative that exhibits 
                     robust folding and enhanced photostability (Zhong et al., 
                     2019)"
                     /label=SiriusGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(340..361)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     948..969
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatcc tctagtactt ctcgacaagc ttggtaccgc atgcctcgag tgcggcccct
      181 aggagatcat gaggccccta ggagatcatg aggccgcaag cttgtcgacg gcgctcgaat
      241 tcggatccgt tatccacttc caatattgga ttggaagtac aggttttcct cgatcccctt
      301 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
      361 gtgatcgcgc ttctcgttgg ggtctttgct cagcttggac tgggtgctca ggtagtggtt
      421 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
      481 ctgcacgctg ccgtcctcga cgttgtggcg gatcttgaag ttcgccttga tgccgttctt
      541 ctgcttgtcg gccgtgatat agacgttgtg gctgttgaag ttgtactcca gcttgtgccc
      601 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
      661 gtcgccctcg aacttcacct cggcgcgggt cttgtaggtg ccgtcgtcct tgaaggagat
      721 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
      781 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg
      841 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgttggt
      901 ggcatcgccc tcgccctcgc cgcgcacgct gaacttgtgg ccgtttacgt cgccgtccag
      961 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca c
//
