LOCUS       sequence-71607-e        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac LIC cloning vector with BioBrick
            poly...
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(411..428)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        join(812..817,818..834,835..840)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatcc tctagtactt ctcgacaagc ttggtaccgc atgcctcgag actgcaggct
      181 ctagattcga aagcggcccc taggagatca tgaggccgcg actagtgagc tcgtcgacgt
      241 aggcctttga attccgcgcg cttcggaccg gttatccact tccaatattg gattggaagt
      301 acttaataaa ggatccgcgc ccgatggtgg gacggtatga ataatccgga atagttatag
      361 gtttttttat tacaaaactg ttacgaaaac agtaaaatac ttatttattt gcgagatggt
      421 tatcattttt aattaagcgg ccgctaatta tctccatgat ctattaatag tccggagtat
      481 acgtagccaa ccactagaac tatagctaga gtcctgggcg aacaaacgat gctcgccttc
      541 cagaaaaccg aggatgcgaa ccacttcatc cggggtcagc accaccggca agcgccgcga
      601 cggccgaggt cttccgatct cctgaagcca gggcagatcc gtgcacagca ccttgccgta
      661 gaagaacagc aaggccgcca atgcctgacg atgcgtggag accgaaacct tgcgctcgtt
      721 cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac
      781 accgtggaaa cggatgaagg cacgaaccca gttgacataa gcctgttcgg ttcgtaaact
      841 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
      901 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg tacagtctat
      961 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt c
//
