LOCUS       sequence-71608-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac His6 TEV cloning vector with BioBrick...
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(268..288)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(289..306)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(426..443)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     promoter        join(827..832,833..849,850..855)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
       61 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      121 attatgatcc tctagtactt ctcgacaagc ttggtaccgc atgcctcgag tgcggcccct
      181 aggagatcat gaggccccta ggagatcatg aggccgcaag cttgtcgacg gcgctcgaat
      241 tcggatccgt tatccacttc caatattgga ttggaagtac aggttttcat ggtgatggtg
      301 atggtgagaa gaacccatcg gaccgggatc cgcgcccgat ggtgggacgg tatgaataat
      361 ccggaatagt tataggtttt tttattacaa aactgttacg aaaacagtaa aatacttatt
      421 tatttgcgag atggttatca tttttaatta agcggccgct aattatctcc atgatctatt
      481 aatagtccgg agtatacgta gccaaccact agaactatag ctagagtcct gggcgaacaa
      541 acgatgctcg ccttccagaa aaccgaggat gcgaaccact tcatccgggg tcagcaccac
      601 cggcaagcgc cgcgacggcc gaggtcttcc gatctcctga agccagggca gatccgtgca
      661 cagcaccttg ccgtagaaga acagcaaggc cgccaatgcc tgacgatgcg tggagaccga
      721 aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg acttcgctgc tgcccaaggt
      781 tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagttga cataagcctg
      841 ttcggttcgt aaactgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt
      901 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt
      961 ttttgtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg
     1021 ttt
//
