LOCUS       sequence-71611-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and PGK1 promoter controlled expression cassettes with G418 
            resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G1-Km
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(173..196)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(230..636)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 aaatcataaa tcataagaaa ttcgcttatt tagaagtgtc aacaacgtat ctaccaacga
       61 tttgaccctt ttccatcttt tcgtaaattt ctggcaaggt agacaagccg acaaccttga
      121 ttggagactt gaccaaacct ctggcgaaga attgttaatt aagagctcag atcttatcgt
      181 cgtcatcctt gtaatccatc gatactagtg cggccgcttg taattaaaac ttagattaga
      241 ttgctatgct ttctttctaa tgagcaagaa gtaaaaaaag ttgtaataga acaagaaaaa
      301 tgaaactgaa acttgagaaa ttgaagaccg tttattaact taaatatcaa tgggaggtca
      361 tcgaaagaga aaaaaatcaa aaaaaaaaat tttcaagaaa aagaaacgtg ataaaaattt
      421 ttattgcctt tttcgacgaa gaaaaagaaa cgaggcggtc tcttttttct tttccaaacc
      481 tttagtacgg gtaattaacg acaccctaga ggaagaaaga ggggaaattt agtatgctgt
      541 gcttgggtgt tttgaagtgg tacggcgatg cgcggagtcc gagaaaatct ggaagagtaa
      601 aaaaggagta gaaacatttt gaagctatgg tgtgtgcggc cggcctggaa gtaccttcaa
      661 agaatggggt cttatcttgt tttgcaagta ccactgagca ggataataat agaaatgata
      721 atatactata gtagagataa cgtcgatgac ttcccatact gtaattgctt ttagttgtgt
      781 atttttagtg tgcaagtttc tgtaaatcga ttaattt
//
