LOCUS       sequence-71613-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and PGK1 promoter controlled expression cassettes with G418 
            resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G2-Km
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(173..196)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aaatcataaa tcataagaaa ttcgcttatt tagaagtgtc aacaacgtat ctaccaacga
       61 tttgaccctt ttccatcttt tcgtaaattt ctggcaaggt agacaagccg acaaccttga
      121 ttggagactt gaccaaacct ctggcgaaga attgttaatt aagagctcag atcttatcgt
      181 cgtcatcctt gtaatccatc gatactagtg cggccgcttg ttttatattt gttgtaaaaa
      241 gtagataatt acttccttga tgatctgtaa aaaagagaaa aagaaagcat ctaagaactt
      301 gaaaaactac gaattagaaa agaccaaata tgtatttctt gcattgacca atttatgcaa
      361 gtttatatat atgtaaatgt aagtttcacg aggttctact aaactaaacc acccccttgg
      421 ttagaagaaa agagtgtgtg agaacaggct gttgttgtca cacgattcgg acaattctgt
      481 ttgaaagaga gagagtaaca gtacgatcga acgaactttg ctctggagat cacagtgggc
      541 atcatagcat gtggtactaa accctttccc gccattccag aaccttcgat tgcttgttac
      601 aaaacctgtg agccgtcgct aggaccttgt tgtgtgacga aattggaagc tgcaatcaat
      661 aggaagacag gaagtcgagc gtgtctgggt tttttcagtt ttgttctttt tgcaaacaaa
      721 tcacgagcga cggtaatttc tttctcgata agaggccacg tgctttatga gggtaacatc
      781 aattcaagaa ggagggaaac acttcct
//
