LOCUS       sequence-71616-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and GAL10 promoter controlled expression cassettes with G418 
            resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G3-Km
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(173..196)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    418..535
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 aaatcataaa tcataagaaa ttcgcttatt tagaagtgtc aacaacgtat ctaccaacga
       61 tttgaccctt ttccatcttt tcgtaaattt ctggcaaggt agacaagccg acaaccttga
      121 ttggagactt gaccaaacct ctggcgaaga attgttaatt aagagctcag atcttatcgt
      181 cgtcatcctt gtaatccatc gatactagtg aattttcaaa aattcttact ttttttttgg
      241 atggacgcaa agaagtttaa taatcatatt acatggcatt accaccatat acatatccat
      301 atctaatctt acttatatgt tgtggaaatg taaagagccc cattatctta gcctaaaaaa
      361 accttctctt tggaactttc agtaatacgc ttaactgctc attgctatat tgaagtacgg
      421 attagaagcc gccgagcggg cgacagccct ccgacggaag actctcctcc gtgcgtcctc
      481 gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa
      541 taaagattct acaatactag cttttatggt tatgaagagg aaaaattggc agtaacctgg
      601 ccccacaaac cttcaaatta acgaatcaaa ttaacaacca taggatgata atgcgattag
      661 ttttttagcc ttatttctgg ggtaattaat cagcgaagcg atgatttttg atctattaac
      721 agatatataa atggaaaagc tgcataacca ctttaactaa tactttcaac attttcagtt
      781 tgtattactt cttattcaaa tgtcataaaa gtatcaaca
//
