LOCUS       sequence-71617-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encode NmCas9 with two NLS and a HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, 
            Thomson JA
  TITLE     Efficient genome engineering in human pluripotent stem cells using 
            Cas9 from Neisseria meningitidis.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 12.
  PUBMED    23940360
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Aug 12."
COMMENT     Sequence Label: pSimpleII-NLS-NmCas9-HA-NLS(s)
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..47)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ttcttcgctg ctggagctgc agcgtaatct ggaacatcgt atgggtaacg gacaggcggg
       61 cgttttttca gacggcatgg tctgatttct ttgcccagtt cgtcaatttg gtatttttgg
      121 aatgaaaggg cggttttgac gccgatacct tccagtattc catttttgcc aattttatga
      181 tcaagatcat gaatgcgtat attgatatta cctgtgcctc gatggcagct ggcaaagtaa
      241 ccaaacattc tagctttttt tgttataacc tcgactaaat cattagggtg taatgagaat
      301 ttaaagttga aactatcatc aataagttgc caatcttctt catcttttcc ttgtacaaca
      361 gccctatccg gcaaaatccc tttcgctacc tgccaactgt aaatcggtac cagataatac
      421 ttgtcgcctt tctcaaacac atctacgcgc accatggttg cgttgtcggc aataccgtta
      481 tggttgcgca cccatacgcc ggttttctgt acttgctcta cgcgtacggc ttttacctgt
      541 tgggtgcggt tgcctgcttt atcgtatttg taaaacggct cggcaaaggc tttggcagga
      601 tcgtctttat gtgcttccag ccgtgctttc agtgcttcgt atagcttagg ttcgcgctcc
      661 cgattgacca ttttttccaa gtctttcagt tttaactgtg tcagcggtac gcgcaacacg
      721 ctgacgcctt cgtccagtcg tttggcggat ttgacggtct ccatatgccc ttgcccgctc
      781 atcttccgat tgggcgcgcg tgaaacaaac agtggcgtaa cgtattcgtg tacggcttcg
      841 gggcgagatg ataatttttc ggcaagcaac gtgcgcagtt tttctagggt atcggcttct
      901 tcgaattcgg gtttgccgtc cgg
//
