LOCUS       sequence-71622-e        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  TITLE     Series-9: N- or C-terminal Tag E.coli T7 Expression Vectors with 
            Biobrick Polycistronic Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET LIC cloning vector with BioBrick polycistr...
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             159..181
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(299..317)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      313..360
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(727..732,733..749,750..755))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(782..800)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        868..972
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
       61 aattaatacg actcactata gggagaccac aacggtttcc ctctagtgcc ggctccggag
      121 agctctttaa tgcggccgct taattaacta gaaataattt tgtttaactt taagaaggag
      181 atatagatat cccaactcca taaggatcgc gatcgccctg caggccggcc ggtaccacgc
      241 gtgcgcgctg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      301 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      361 ctgaaaggag gaactatatc cggattatcc acaggacggg tgtggtcgcc atgatcgcgt
      421 agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg
      481 acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca
      541 cgccatagtg actggcgatg ctgtcggaat ggacgaaatc ccgcaagagg cccggcagta
      601 ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga
      661 gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac
      721 taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag
      781 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      841 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      901 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataacattga
      961 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     1021 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     1081 cagttgggtg cacgagtggg ttacatcgaa ctggatctca
//
