LOCUS       sequence-71623-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E.coli genomic integration at lacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Sabri S, Steen JA, Bongers M, Nielsen LK, Vickers CE
  TITLE     Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA
            sequences, including whole metabolic pathways, onto the Escherichia 
            coli chromosome at well-characterised loci.
  JOURNAL   Microb Cell Fact. 2013 Jun 24;12(1):60.
  PUBMED    23799955
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-06-24"; volume: "12(1)"; pages: "60"
COMMENT     Sequence Label: pKIKOlacZKm
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    278..325
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg
       61 cttgctgtcc ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc
      121 tgctttctct ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc
      181 cggggtcagc accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc
      241 ttgcgccctg agtgcttgcg gcagcgtggg ggatcttgaa gttcctattc cgaagttcct
      301 attctctaga aagtatagga acttcgaagc agctccagcc tacgattaaa aaaaaccccg
      361 ccgaagcggg gttttttttg tttatcctgc tgatgaagca gaacaacttt aacgccgtgc
      421 gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt
      481 atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg
      541 atgatccgcg ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc
      601 gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc
      661 acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag
      721 gcggcggagc cgacaccacg gccaccgata ttatt
//
