LOCUS       sequence-71626-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and PGK1 promoter controlled expression cassettes with 
            phleomycin resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G2-Ph
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      26..191
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(81..105)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(214..247)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(310..507)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 atacgttgtt gacacttcta aataagcgaa tttcttatga tttatgattt ttattattaa
       61 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg
      121 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca
      181 tgaggtcgct cctagtggat ctgatatcac ctaataactt cgtatagcat acattatacg
      241 aagttatatt aagggttctc gagagctcgt tttcgacact ggatggcggc gttagtatcg
      301 aatcgacagc agtatagcga ccagcattca catacgattg acgcatgata ttactttctg
      361 cgcacttaac ttcgcatctg ggcagatgat gtcgaggcga aaaaaaatat aaatcacgct
      421 aacatttgat taaaatagaa caactacaat ataaaaaaac tatacaaatg acaagttctt
      481 gaaaacaaga atctttttat tgtcagtact gatcatgaga tgcctgcaag caattcgttc
      541 tgtatcaggc gcaggagcgt cccgtccggg tcgaccaaag cggccatcgt gcctccccac
      601 tcctgcagtt cgggggcatg gatgcgcgga tagccgctgc tggtttcctg gatgccgacg
      661 gatttgcact gccggtagaa ctccgcgagg tcgtccagcc tcaggcagca gctgaaccaa
      721 ctcgcgaggg gatcgagccc ggggtgggcg aagaactcca gcatgagatc cccgcgctgg
      781 aggatcatcc agccggcgtc cc
//
