LOCUS       sequence-71628-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and GAL10 promoter controlled expression cassettes with G418 
            resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G3-Km
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      29..194
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(84..108)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(217..250)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(313..510)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 tagatacgtt gttgacactt ctaaataagc gaatttctta tgatttatga tttttattat
       61 taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa
      121 acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata
      181 gcatgaggtc gctcctagtg gatctgatat cacctaataa cttcgtatag catacattat
      241 acgaagttat attaagggtt ctcgagagct cgttttcgac actggatggc ggcgttagta
      301 tcgaatcgac agcagtatag cgaccagcat tcacatacga ttgacgcatg atattacttt
      361 ctgcgcactt aacttcgcat ctgggcagat gatgtcgagg cgaaaaaaaa tataaatcac
      421 gctaacattt gattaaaata gaacaactac aatataaaaa aactatacaa atgacaagtt
      481 cttgaaaaca agaatctttt tattgtcagt actgattaga aaaactcatc gagcatcaaa
      541 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
      601 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
      661 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
      721 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc
      781 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaa
//
