LOCUS       sequence-71629-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and GAL10 promoter controlled expression cassettes with 
            phleomycin resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G3-Ph
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      31..196
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(86..110)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(219..252)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(315..512)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat gatttttatt
       61 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
      121 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
      181 tagcatgagg tcgctcctag tggatctgat atcacctaat aacttcgtat agcatacatt
      241 atacgaagtt atattaaggg ttctcgagag ctcgttttcg acactggatg gcggcgttag
      301 tatcgaatcg acagcagtat agcgaccagc attcacatac gattgacgca tgatattact
      361 ttctgcgcac ttaacttcgc atctgggcag atgatgtcga ggcgaaaaaa aatataaatc
      421 acgctaacat ttgattaaaa tagaacaact acaatataaa aaaactatac aaatgacaag
      481 ttcttgaaaa caagaatctt tttattgtca gtactgatca tgagatgcct gcaagcaatt
      541 cgttctgtat caggcgcagg agcgtcccgt ccgggtcgac caaagcggcc atcgtgcctc
      601 cccactcctg cagttcgggg gcatggatgc gcggatagcc gctgctggtt tcctggatgc
      661 cgacggattt gcactgccgg tagaactccg cgaggtcgtc cagcctcagg cagcagctga
      721 accaactcgc gaggggatcg agcccggggt gggcgaagaa ctccagcatg agatccccgc
      781 gctggaggat catccagccg gcg
//
