LOCUS       sequence-71634-e        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  TITLE     Series-9: N- or C-terminal Tag E.coli T7 Expression Vectors with 
            Biobrick Polycistronic Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 MBP TEV cloning vector with BioBrick
            ...
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             159..181
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             201..218
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
       61 aattaatacg actcactata gggagaccac aacggtttcc ctctagtgcc ggctccggag
      121 agctctttaa tgcggccgct taattaacta gaaataattt tgtttaactt taagaaggag
      181 atatacatat gaaatcttct caccatcacc atcaccatgg ttcttctatg aaaatcgaag
      241 aaggtaaact ggtaatctgg attaacggcg ataaaggcta taacggtctc gctgaagtcg
      301 gtaagaaatt cgagaaagat accggaatta aagtcaccgt tgagcatccg gataaactgg
      361 aagagaaatt cccacaggtt gcggcaactg gcgatggccc tgacattatc ttctgggcac
      421 acgaccgctt tggtggctac gctcaatctg gcctgttggc tgaaatcacc ccggacaaag
      481 cgttccagga caagctgtat ccgtttacct gggatgccgt acgttacaac ggcaagctga
      541 ttgcttaccc gatcgctgtt gaagcgttat cgctgattta taacaaagat ctgctgccga
      601 acccgccaaa aacctgggaa gagatcccgg cgctggataa agaactgaaa gcgaaaggta
      661 agagcgcgct gatgttcaac ctgcaagaac cgtacttcac ctggccgctg attgctgctg
      721 acgggggtta tgcgttcaag tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg
      781 ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa aacaaacaca
      841 tgaatgcaga caccgattac tccatcgcag aagctgcctt taataaaggc gaaacagcga
      901 tgaccatcaa cggcccgtgg gcatggtcca acatcgacac cagcaaagtg aattatggtg
      961 taacggtact gccgaccttc aagggtcaac catccaaacc gttcgttggc gtgctgagcg
     1021 caggtattaa cgccgccagt ccgaacaaag agctggcaaa agagttcctc gaaaactatc
     1081 tgctgactga tgaaggtctg gaagcggtta ataaagacaa
//
