LOCUS       sequence-71635-e        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  TITLE     Series-9: N- or C-terminal Tag E.coli T7 Expression Vectors with 
            Biobrick Polycistronic Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 MBP TEV cloning vector with BioBrick
            ...
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(98..118)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(176..199)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 gcttcctttc gggctttgtt agcagccgga tcagcgcgca cgcgtggtac cggccggcct
       61 gcagggcgat cgcgatccgt tatccacttc caatattgga ttggaagtac aggttttcct
      121 cgatcccgag gttgttgtta ttgttattgt tgttgttgtt cgagctcgaa ttagtctgcg
      181 cgtctttcag ggcttcatcg acagtctgac gaccgctggc ggcgttgatc accgcagtac
      241 gcacggcata ccagaaagcg gacatctgcg ggatgttcgg catgatttca cctttctggg
      301 cgttttccat agtggcggca atacgtggat ctttcgccaa ctcttcctcg taagacttca
      361 gcgctacggc acccagcggt ttgtctttat taaccgcttc cagaccttca tcagtcagca
      421 gatagttttc gaggaactct tttgccagct ctttgttcgg actggcggcg ttaatacctg
      481 cgctcagcac gccaacgaac ggtttggatg gttgaccctt gaaggtcggc agtaccgtta
      541 caccataatt cactttgctg gtgtcgatgt tggaccatgc ccacgggccg ttgatggtca
      601 tcgctgtttc gcctttatta aaggcagctt ctgcgatgga gtaatcggtg tctgcattca
      661 tgtgtttgtt tttaatcagg tcaaccagga aggtcagacc cgctttcgcg ccagcgttat
      721 ccacgcccac gtctttaatg tcgtacttgc cgttttcata cttgaacgca taacccccgt
      781 cagcagcaat cagcggccag gtgaagtacg gttcttgcag gttgaacatc agcgcgctct
      841 tacctttcgc tttcagttct ttatccagcg ccgggatctc ttcccaggtt tttggcgggt
      901 tcggcagcag atctttgtta taaatcagcg ataacgcttc aacagcgatc gggtaagcaa
      961 tcagcttgcc gttgtaacgt acggcatccc aggtaaacgg atacagcttg
//
