LOCUS       sequence-71636-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ads with trxA tag under Pgrac promoter (inducible by IPTG)
            in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Zhou K, Zou R, Zhang C, Stephanopoulos G, Too HP
  TITLE     Optimization of amorphadiene synthesis in Bacillus subtilis via 
            transcriptional, translational, and media modulation.
  JOURNAL   Biotechnol Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900.
  PUBMED    23483530
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900."
COMMENT     Sequence Label: pHT-trxA.ads
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    361..385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             407..733
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
ORIGIN
        1 atatttgatt gatcgtaacc agatgaagca ctctttccac tatccctaca gtgttatggc
       61 ttgaacaatc acgaaacaat aattggtacg tacgatcttt cagccgactc aaacatcaaa
      121 tcttacaaat gtagtctttg aaagtattac atatgtaaga tttaaatgca accgtttttt
      181 cggaaggaaa tgatgacctc gtttccaccg gaattagctt ggtaccagct attgtaacat
      241 aatcggtacg ggggtgaaaa agctaacgga aaagggagcg gaaaagaatg atgtaagcgt
      301 gaaaaatttt ttatcttatc acttgaaatt ggaagggaga ttctttatta taagaattgt
      361 ggaattgtga gcggataaca attcccaatt aaaggaggaa ggatccatga gcgataaaat
      421 tattcacctg actgacgaca gttttgacac ggatgtactc aaagcggacg gggcgatcct
      481 cgtcgatttc tgggcagagt ggtgcggtcc gtgcaaaatg atcgccccga ttctggatga
      541 aatcgctgac gaatatcagg gcaaactgac cgttgcaaaa ctgaacatcg atcaaaaccc
      601 tggcactgcg ccgaaatatg gcatccgtgg tatcccgact ctgctgctgt tcaaaaacgg
      661 tgaagtggcg gcaaccaaag tgggtgcact gtctaaaggt cagttgaaag agttcctcga
      721 cgctaacctg gcgggctcag gcggcggcgg ctcagagctc tctctgactg aggaaaaacc
      781 aatccgtccg atcgcaaact ttccgccgag catctgg
//
