LOCUS       sequence-71638-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ads under Pgrac promoter (inducible by IPTG) and expresses
            dxs and idi under PxylA promoter (inducible by xylose) in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Zhou K, Zou R, Zhang C, Stephanopoulos G, Too HP
  TITLE     Optimization of amorphadiene synthesis in Bacillus subtilis via 
            transcriptional, translational, and media modulation.
  JOURNAL   Biotechnol Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900.
  PUBMED    23483530
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900."
COMMENT     Sequence Label: pHT-6xR.ads-PxylA-DI
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    358..382
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tttgattgat cgtaaccaga tgaagcactc tttccactat ccctacagtg ttatggcttg
       61 aacaatcacg aaacaataat tggtacgtac gatctttcag ccgactcaaa catcaaatct
      121 tacaaatgta gtctttgaaa gtattacata tgtaagattt aaatgcaacc gttttttcgg
      181 aaggaaatga tgacctcgtt tccaccggaa ttagcttggt accagctatt gtaacataat
      241 cggtacgggg gtgaaaaagc taacggaaaa gggagcggaa aagaatgatg taagcgtgaa
      301 aaatttttta tcttatcact tgaaattgga agggagattc tttattataa gaattgtgga
      361 attgtgagcg gataacaatt cccaattaaa ggaggaagga tccatgagac gcagacgcag
      421 aagatctctg actgaggaaa aaccaatccg tccgatcgca aactttccgc cgagcatctg
      481 gggtgatcag ttcctgatct accagaagca ggtcgagcaa ggcgtagaac agatcgtgaa
      541 cgacctgaaa aaggaggtac gtcagctgct gaaagaggct ctggacatcc cgatgaaaca
      601 tgcgaacctg ctgaagctga ttgatgagat tcagcgcctg ggtatcccat accacttcga
      661 acgtgaaatt gatcacgcgc tgcagtgtat ttatgaaacg tatggtgata actggaacgg
      721 tgaccgtagc tccctgtggt tccgtctgat gcgtaagcag ggttattacg tgacctgcga
      781 cgtcttcaac aactacaaag acaagaacg
//
