LOCUS       sequence-71642-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ads with trxA tag under Pgrac promoter (inducible by IPTG)
            in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Zhou K, Zou R, Zhang C, Stephanopoulos G, Too HP
  TITLE     Optimization of amorphadiene synthesis in Bacillus subtilis via 
            transcriptional, translational, and media modulation.
  JOURNAL   Biotechnol Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900.
  PUBMED    23483530
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900."
COMMENT     Sequence Label: pHT-trxA.ads
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(161..178)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gactattcct aataagccga tattagcctc gtatgtttca accatttgtt ccaggtaagg
       61 tataaacttt tcagttgcag acaaagatct ccatggacgc gtgacgtgaa aaaagcccgc
      121 tcattaggcg ggctgccccg gggacgtcga ctctagatca gtgatggtga tgatgatgga
      181 tggacattgg gtaaaccagc agggacttga tcaggtgttt gtattcgtca cccatacggg
      241 tgaagttatc tttgcccgca tactgcactt ccagaaactg gcacagataa atcactgcca
      301 tcagcagcgg gcgtgggatg tttttagtag tcaggtactc acggttgatg tctttccata
      361 catcctcaac ctctttgtag atcagggtct gcgcatactc ttcgttaacg ttgtattctt
      421 tcatgtaaga ttccagagag ctgctggagt gtttacgttc ctgctcggct ttatgggtca
      481 tcagatcgtt caggcgacgg cccaggatac cagagtaacg gaacagcgga ggcgcagaca
      541 ctgcccattc aacgctttct ttcgtaaaaa tgtcggacat acccaggtag caagtggtag
      601 tcagcaggtt tgcgccaccg gtgatgataa ctaccgggtc gtgttcttcg gtggtcggga
      661 tgtgaccctc gtttgcccac ttcgcctcca ccatcaggtt gcggacgaat tctttcacaa
      721 actccttgcc acagttgaac agatcagtgc gaccttcttt tgccaggaac tcttccattt
      781 cggtataagt atccatgaac agtttataga tcggtttcat
//
