LOCUS       sequence-71648-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ads under Pgrac promoter (inducible by IPTG) and expresses
            dxs and idi under PxylA promoter (inducible by xylose) in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Zhou K, Zou R, Zhang C, Stephanopoulos G, Too HP
  TITLE     Optimization of amorphadiene synthesis in Bacillus subtilis via 
            transcriptional, translational, and media modulation.
  JOURNAL   Biotechnol Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900.
  PUBMED    23483530
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900."
COMMENT     Sequence Label: pHT-6xR.ads-PxylA-DI
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(233..250)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agtgaacgca aaggttagca aactatatca cgtttgatct taagaatgaa ggagctactt
       61 tcaattaatt gattttctat aaaagagaaa gaattaccct cttctattaa gagtgttatg
      121 tcccaggtaa ggtataaact tttcagttgc agacaaagat ctccatggac gcgtgacgtg
      181 aaaaaagccc gctcattagg cgggctgccc cggggacgtc gactctagat cagtgatggt
      241 gatgatgatg gatggacatt gggtaaacca gcagggactt gatcaggtgt ttgtattcgt
      301 cacccatacg ggtgaagtta tctttgcccg catactgcac ttccagaaac tggcacagat
      361 aaatcactgc catcagcagc gggcgtggga tgtttttagt agtcaggtac tcacggttga
      421 tgtctttcca tacatcctca acctctttgt agatcagggt ctgcgcatac tcttcgttaa
      481 cgttgtattc tttcatgtaa gattccagag agctgctgga gtgtttacgt tcctgctcgg
      541 ctttatgggt catcagatcg ttcaggcgac ggcccaggat accagagtaa cggaacagcg
      601 gaggcgcaga cactgcccat tcaacgcttt ctttcgtaaa aatgtcggac atacccaggt
      661 agcaagtggt agtcagcagg tttgcgccac cggtgatgat aactaccggg tcgtgttctt
      721 cggtggtcgg gatgtgaccc tcgtttgccc acttcgcctc caccatcagg ttgcggacga
      781 attctttcac aaactccttg ccacagttga acagatcagt gcgaccttct tt
//
