LOCUS       sequence-71650-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  TITLE     Series-9: N- or C-terminal Tag E.coli T7 Expression Vectors with 
            Biobrick Polycistronic Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 Mocr TEV cloning vector with BioBrick...
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             159..181
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             201..218
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             588..608
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(723..741)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      737..784
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
       61 aattaatacg actcactata gggagaccac aacggtttcc ctctagtgcc ggctccggag
      121 agctctttaa tgcggccgct taattaacta gaaataattt tgtttaactt taagaaggag
      181 atatacatat gaaatcttct caccatcacc atcaccatgg ttcttctatg agcaacatga
      241 catacaataa tgttttcgac cacgcgtacg aaatgcttaa ggagaacatc cgttatgatg
      301 acatacggga tacagatgac cttcacgacg caattcatat ggccgccgat aatgccgtcc
      361 ctcactatta tgccgatatt cggtcagtta tggcatctga aggtattgat ctggaatttg
      421 aggatagtgg cctcatgcca gatacgaagg acgacatcag aattctgcaa gcgcggatct
      481 acgaacaact taccatcgat ttatgggaag atgcggaaga tctcctgaat gaatacctgg
      541 aagaagtgga ggagtatgag gaagatgaag aggggacggg gatcgaggaa aacctgtact
      601 tccaatccaa tattggaagt ggataacgga tcgcgatcgc cctgcaggcc ggccggtacc
      661 acgcgtgcgc gctgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
      721 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      781 tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg
      841 cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaa
//
