LOCUS       sequence-71655-e        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  TITLE     Series-13: Co-transformation vectors for Series-2 transfer vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 Sumo TEV co-transformation cloning ve...
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     118..137
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        118..136
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    141..164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             217..239
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             259..276
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             295..588
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             595..615
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(740..758)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      754..801
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca
       61 ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaaattaa
      121 tacgactcac tatagggaga gaattgtgag cggataacaa ttcccctgta gattaattaa
      181 gcggccgccc tgcaggactc gagttctaga aataattttg tttaacttta agaaggagat
      241 atacatatga aatcttctca ccatcaccat caccatggtt cttctatggc tagcatgtcg
      301 gactcagaag tcaatcaaga agctaagcca gaggtcaagc cagaagtcaa gcctgagact
      361 cacatcaatt taaaggtgtc cgatggatct tcagagatct tcttcaagat caaaaagacc
      421 actcctttaa gaaggctgat ggaagcgttc gctaaaagac agggtaagga aatggactcc
      481 ttaagattct tgtacgacgg tattagaatt caagctgatc agacccctga agatttggac
      541 atggaggata acgatattat tgaggctcac agagaacaga ttggtgggat cgaggaaaac
      601 ctgtacttcc aatccaatat tggaagtgga taacggatcc gcgatcgcgg cgcgccacct
      661 ggtggccggc cggtaccacg cgtgcgcgct gatccggctg ctaacaaagc ccgaaaggaa
      721 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
      781 cgggtcttga ggggtttttt gctgaaacct caggcatttg agaagcacac ggtcacactg
      841 cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc
      901 tgccctgaac cgacgaccgg gtcatcgtgg ccggatcttg cggcccctcg gcttgaacga
      961 attgttagac attatttgcc gactaccttg gtgatctcgc ctttcacgta atggacaaat
     1021 tcttccaact gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt
     1081 ctagct
//
