LOCUS       sequence-71661-e        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  TITLE     Series-14: N-terminal Tag E.coli T7 Expression Vectors with Biobrick
            POLYPROMOTER Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 Thioredoxin TEV expression vector wit...
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      61..88
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        114..132
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             149..171
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             190..207
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             208..531
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="SDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEI
                     ADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLD
                     ANLA"
     CDS             541..561
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      603..649
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctgttgttt gtcggtgagt ccttccagtc tgggcgaaag cgcggtcgat caaattaagc
       61 agaaggccat cctgacggat ggcctttttg cgtttctatc gatcccgcga aattaatacg
      121 actcactata ggcccctcta gaaataattt tgtttaactt taagaaggag atataccatg
      181 ggttcttctc accatcacca tcaccatagc gataaaatta ttcacctgac tgacgacagt
      241 tttgacacgg atgtactcaa agcggacggg gcgatcctcg tcgatttctg ggcagagtgg
      301 tgcggtccgt gcaaaatgat cgccccgatt ctggatgaaa tcgctgacga atatcagggc
      361 aaactgaccg ttgcaaaact gaacatcgat caaaaccctg gcactgcgcc gaaatatggc
      421 atccgtggta tcccgactct gctgctgttc aaaaacggtg aagtggcggc aaccaaagtg
      481 ggtgcactgt ctaaaggtca gttgaaagag ttcctcgacg ctaacctggc cgggatcgag
      541 gaaaacctgt acttccaatc caatattgga agtggataac tggctgctgc caccgctgag
      601 catagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg
      661 aactatatca agtcccgaag tagctgtcag actgctggcg accgccgcaa ctagtggcgt
      721 catgcgtgcc gtatggtctt tcgcctgtag acgccctaca agccgtacta aagtggaaag
      781 acccgcaaaa ggtatcgcga tcgccctgca gggaactata tccgactagt agcccgccta
      841 atgagcgggc ttttttttaa ttcccctatt tgtttatttt tctaaataca ttcaaatatg
      901 tatccgctca tgagacaata accctgataa atgcttcgaa aaaggaagag tatgagtatt
      961 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1021 cacccagaaa cgctcgtgaa agtaaaagac gc
//
