LOCUS       sequence-71666-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  TITLE     Series-13: Co-transformation vectors for Series-2 transfer vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET StrepII TEV co-transformation cloning vect...
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     125..144
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        125..143
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             232..254
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             271..294
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             301..321
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(446..464)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      460..507
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      121 aaattaatac gactcactat agggagacca caacggtttc cctctagtgc cggctccgga
      181 gagctcttta attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa
      241 ctttaagaag gagatataca tatggctagc tggagccacc cgcagttcga aaaaggcgcc
      301 gaaaacctgt acttccaatc caatattgga agtggataac ggatccgcga tcgcggcgcg
      361 ccacctggtg gccggccggt accacgcgtg cgcgctgatc cggctgctaa caaagcccga
      421 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      481 tctaaacggg tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc
      541 acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa
      601 cgaccctgcc ctgaaccgac gacaagctga cgaccgggtc tccgcaagtg gcacttttcg
      661 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      721 gctcatgaat taattcttag aaaaactcat cgagcatcaa atgaaactgc aatttattca
      781 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa gg
//
