LOCUS       sequence-71670-e        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  TITLE     Series-13: Co-transformation vectors for Series-2 transfer vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 MBP TEV co-transformation cloning vec...
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(184..202)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      198..245
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 acaacaacaa taacaataac aacaacctcg ggatcgagga aaacctgtac ttccaatcca
       61 atattggaag tggataacgg atccgcgatc gcggcgcgcc acctggtggc cggccggtac
      121 cacgcgtgcg cgctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg
      181 ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt
      241 ttttgctgaa acctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata
      301 aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga
      361 ccgggtcatc gtggccggat cttgcggccc ctcggcttga acgaattgtt agacattatt
      421 tgccgactac cttggtgatc tcgcctttca cgtaatggac aaattcttcc aactgatctg
      481 cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga
      541 cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg
      601 cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct
      661 catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa
      721 atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa
      781 cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct
      841 cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag
      901 ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga
      961 gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc
     1021 gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgcggct
     1081 tcaggcc
//
