LOCUS       sequence-71672-e        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  TITLE     Series-9: N- or C-terminal Tag E.coli T7 Expression Vectors with 
            Biobrick Polycistronic Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 Sumo TEV cloning vector with BioBrick...
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             159..181
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             201..218
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             237..530
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             537..557
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(672..690)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      686..733
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
       61 aattaatacg actcactata gggagaccac aacggtttcc ctctagtgcc ggctccggag
      121 agctctttaa tgcggccgct taattaacta gaaataattt tgtttaactt taagaaggag
      181 atatacatat gaaatcttct caccatcacc atcaccatgg ttcttctatg gctagcatgt
      241 cggactcaga agtcaatcaa gaagctaagc cagaggtcaa gccagaagtc aagcctgaga
      301 ctcacatcaa tttaaaggtg tccgatggat cttcagagat cttcttcaag atcaaaaaga
      361 ccactccttt aagaaggctg atggaagcgt tcgctaaaag acagggtaag gaaatggact
      421 ccttaagatt cttgtacgac ggtattagaa ttcaagctga tcagacccct gaagatttgg
      481 acatggagga taacgatatt attgaggctc acagagaaca gattggtggg atcgaggaaa
      541 acctgtactt ccaatccaat attggaagtg gataacggat cgcgatcgcc ctgcaggccg
      601 gccggtacca cgcgtgcgcg ctgatccggc tgctaacaaa gcccgaaagg aagctgagtt
      661 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      721 gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg
      781 ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc
      841 caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata
      901 gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga
      961 ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga
     1021 ggatgacgat gagcgcattg
//
