LOCUS       sequence-71673-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  TITLE     Series-9: N- or C-terminal Tag E.coli T7 Expression Vectors with 
            Biobrick Polycistronic Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 Thioredoxin TEV cloning vector with
            B...
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             159..181
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             201..218
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             228..554
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             564..584
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(699..717)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      713..760
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
       61 aattaatacg actcactata gggagaccac aacggtttcc ctctagtgcc ggctccggag
      121 agctctttaa tgcggccgct taattaacta gaaataattt tgtttaactt taagaaggag
      181 atatacatat gaaatcttct caccatcacc atcaccatgg ttcttctatg agcgataaaa
      241 ttattcacct gactgacgac agttttgaca cggatgtact caaagcggac ggggcgatcc
      301 tcgtcgattt ctgggcagag tggtgcggtc cgtgcaaaat gatcgccccg attctggatg
      361 aaatcgctga cgaatatcag ggcaaactga ccgttgcaaa actgaacatc gatcaaaacc
      421 ctggcactgc gccgaaatat ggcatccgtg gtatcccgac tctgctgctg ttcaaaaacg
      481 gtgaagtggc ggcaaccaaa gtgggtgcac tgtctaaagg tcagttgaaa gagttcctcg
      541 acgctaacct ggccgggatc gaggaaaacc tgtacttcca atccaatatt ggaagtggat
      601 aacggatcgc gatcgccctg caggccggcc ggtaccacgc gtgcgcgctg atccggctgc
      661 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      721 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      781 cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta
      841 gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg
      901 cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc
      961 tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc
     1021 cta
//
