LOCUS       sequence-71677-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encode NmCas9 with two NLS and a HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, 
            Thomson JA
  TITLE     Efficient genome engineering in human pluripotent stem cells using 
            Cas9 from Neisseria meningitidis.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 12.
  PUBMED    23940360
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Aug 12."
COMMENT     Sequence Label: pSimpleII-NLS-NmCas9-HA-NLS(s)
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             17..37
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caccgccacc atggtgccta agaagaagag aaaggtggct gccttcaaac ctaattcaat
       61 caactacatc ctcggcctcg atatcggcat cgcatccgtc ggctgggcga tggtagaaat
      121 tgacgaagaa gaaaacccca tccgcctgat tgatttgggc gtgcgcgtat ttgagcgtgc
      181 cgaagtaccg aaaacaggcg actcccttgc catggcaagg cgtttggcgc gcagtgttcg
      241 ccgcctgacc cgccgtcgcg cccaccgcct gcttcggacc cgccgcctat tgaaacgcga
      301 aggcgtatta caagccgcca attttgacga aaacggcttg attaaatcct taccgaatac
      361 accatggcaa cttcgcgcag ccgcattaga ccgcaaactg acgcctttag agtggtcggc
      421 agtcttgttg catttaatca aacatcgcgg ctatttatcg caacggaaaa acgagggcga
      481 aactgccgat aaggagcttg gcgctttgct taaaggcgta gccggcaatg cccatgcctt
      541 acagacaggc gatttccgca caccggccga attggcttta aataaatttg agaaagaaag
      601 cggccatatc cgcaatcagc gcagcgatta ttcgcatacg ttcagccgca aagatttaca
      661 ggcggagctg attttgctgt ttgaaaaaca aaaagaattt ggcaatccgc atgtttcagg
      721 cggccttaaa gaaggtattg aaaccctact gatgacgcaa cgccctgccc tgtccggcga
      781 tgccgttcaa aaaatgttgg ggcattgcac cttcgaaccg gcagagccga aagccgctaa
      841 aaacacctac acagccgaac gtttcatctg gctgaccaag ctgaacaacc tgcgtatttt
      901 a
//
