LOCUS       sequence-71682-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one plasmid targeting tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, 
            Thomson JA
  TITLE     Efficient genome engineering in human pluripotent stem cells using 
            Cas9 from Neisseria meningitidis.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 12.
  PUBMED    23940360
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Aug 12."
COMMENT     Sequence Label: 
            pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..49)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ttttcttcgc tgctggagct gcagcgtaat ctggaacatc gtatgggtaa cggacaggcg
       61 ggcgtttttt cagacggcat ggtctgattt ctttgcccag ttcgtcaatt tggtattttt
      121 ggaatgaaag ggcggttttg acgccgatac cttccagtat tccatttttg ccaattttat
      181 gatcaagatc atgaatgcgt atattgatat tacctgtgcc tcgatggcag ctggcaaagt
      241 aaccaaacat tctagctttt tttgttataa cctcgactaa atcattaggg tgtaatgaga
      301 atttaaagtt gaaactatca tcaataagtt gccaatcttc ttcatctttt ccttgtacaa
      361 cagccctatc cggcaaaatc cctttcgcta cctgccaact gtaaatcggt accagataat
      421 acttgtcgcc tttctcaaac acatctacgc gcaccatggt tgcgttgtcg gcaataccgt
      481 tatggttgcg cacccatacg ccggttttct gtacttgctc tacgcgtacg gcttttacct
      541 gttgggtgcg gttgcctgct ttatcgtatt tgtaaaacgg ctcggcaaag gctttggcag
      601 gatcgtcttt atgtgcttcc agccgtgctt tcagtgcttc gtatagctta ggttcgcgct
      661 cccgattgac cattttttcc aagtctttca gttttaactg tgtcagcggt acgcgcaaca
      721 cgctgacgcc ttcgtccagt cgtttggcgg atttgacggt ctccatatgc ccttgcccgc
      781 tcatcttccg attgggcgcg cgtgaaacaa acagtggcgt aacgtattcg tgtacggctt
      841 cggggcgaga tgataatttt tcggcaagca acgtgcgcag tttttct
//
