LOCUS       sequence-71685-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one plasmid targeting tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, 
            Thomson JA
  TITLE     Efficient genome engineering in human pluripotent stem cells using 
            Cas9 from Neisseria meningitidis.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 12.
  PUBMED    23940360
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Aug 12."
COMMENT     Sequence Label: 
            pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..22
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcctaagaag aagagaaagg tggctgcctt caaacctaat tcaatcaact acatcctcgg
       61 cctcgatatc ggcatcgcat ccgtcggctg ggcgatggta gaaattgacg aagaagaaaa
      121 ccccatccgc ctgattgatt tgggcgtgcg cgtatttgag cgtgccgaag taccgaaaac
      181 aggcgactcc cttgccatgg caaggcgttt ggcgcgcagt gttcgccgcc tgacccgccg
      241 tcgcgcccac cgcctgcttc ggacccgccg cctattgaaa cgcgaaggcg tattacaagc
      301 cgccaatttt gacgaaaacg gcttgattaa atccttaccg aatacaccat ggcaacttcg
      361 cgcagccgca ttagaccgca aactgacgcc tttagagtgg tcggcagtct tgttgcattt
      421 aatcaaacat cgcggctatt tatcgcaacg gaaaaacgag ggcgaaactg ccgataagga
      481 gcttggcgct ttgcttaaag gcgtagccgg caatgcccat gccttacaga caggcgattt
      541 ccgcacaccg gccgaattgg ctttaaataa atttgagaaa gaaagcggcc atatccgcaa
      601 tcagcgcagc gattattcgc atacgttcag ccgcaaagat ttacaggcgg agctgatttt
      661 gctgtttgaa aaacaaaaag aatttggcaa tccgcatgtt tcaggcggcc ttaaagaagg
      721 tattgaaacc ctactgatga cgcaacgccc tgccctgtcc ggcgatgccg ttcaaaaaat
      781 gttggggcat tgcaccttcg aaccggcaga gccgaaagcc gctaaaaaca cctacacagc
      841 cgaacgtttc atctggctga ccaagctgaa caacctgcgt attttagagc aaggcagcga
      901 gcggccattg accgataccg aacgcgccac gcttatggac gagccataca gaaaatccaa
//
