LOCUS       sequence-71692-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one plasmid targeting human OCT4, cuts roughly ~84bp 
            downstream of stop codon..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, 
            Thomson JA
  TITLE     Efficient genome engineering in human pluripotent stem cells using 
            Cas9 from Neisseria meningitidis.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 12.
  PUBMED    23940360
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Aug 12."
COMMENT     Sequence Label: 
            pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             18..38
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 acaccgccac catggtgcct aagaagaaga gaaaggtggc tgccttcaaa cctaattcaa
       61 tcaactacat cctcggcctc gatatcggca tcgcatccgt cggctgggcg atggtagaaa
      121 ttgacgaaga agaaaacccc atccgcctga ttgatttggg cgtgcgcgta tttgagcgtg
      181 ccgaagtacc gaaaacaggc gactcccttg ccatggcaag gcgtttggcg cgcagtgttc
      241 gccgcctgac ccgccgtcgc gcccaccgcc tgcttcggac ccgccgccta ttgaaacgcg
      301 aaggcgtatt acaagccgcc aattttgacg aaaacggctt gattaaatcc ttaccgaata
      361 caccatggca acttcgcgca gccgcattag accgcaaact gacgccttta gagtggtcgg
      421 cagtcttgtt gcatttaatc aaacatcgcg gctatttatc gcaacggaaa aacgagggcg
      481 aaactgccga taaggagctt ggcgctttgc ttaaaggcgt agccggcaat gcccatgcct
      541 tacagacagg cgatttccgc acaccggccg aattggcttt aaataaattt gagaaagaaa
      601 gcggccatat ccgcaatcag cgcagcgatt attcgcatac gttcagccgc aaagatttac
      661 aggcggagct gattttgctg tttgaaaaac aaaaagaatt tggcaatccg catgtttcag
      721 gcggccttaa agaaggtatt gaaaccctac tgatgacgca acgccctgcc ctgtccggcg
      781 atgccgttca aaaaatgttg gggcattgca ccttcgaacc ggcagagccg aaagccgcta
      841 aaaacaccta cacagccgaa cgtttcatct ggctgaccaa gctgaacaac ctgcgtattt
      901 t
//
