LOCUS       sequence-71708-e        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  TITLE     Series-13: Co-transformation vectors for Series-2 transfer vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 Thioredoxin TEV co-transformation clo...
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     125..144
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        125..143
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    148..171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             224..246
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             266..283
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             293..619
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             629..649
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(774..792)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      788..835
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      121 aaattaatac gactcactat agggagagaa ttgtgagcgg ataacaattc ccctgtagat
      181 taattaagcg gccgccctgc aggactcgag ttctagaaat aattttgttt aactttaaga
      241 aggagatata catatgaaat cttctcacca tcaccatcac catggttctt ctatgagcga
      301 taaaattatt cacctgactg acgacagttt tgacacggat gtactcaaag cggacggggc
      361 gatcctcgtc gatttctggg cagagtggtg cggtccgtgc aaaatgatcg ccccgattct
      421 ggatgaaatc gctgacgaat atcagggcaa actgaccgtt gcaaaactga acatcgatca
      481 aaaccctggc actgcgccga aatatggcat ccgtggtatc ccgactctgc tgctgttcaa
      541 aaacggtgaa gtggcggcaa ccaaagtggg tgcactgtct aaaggtcagt tgaaagagtt
      601 cctcgacgct aacctggccg ggatcgagga aaacctgtac ttccaatcca atattggaag
      661 tggataacgg atccgcgatc gcggcgcgcc acctggtggc cggccggtac cacgcgtgcg
      721 cgctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      781 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      841 acctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa
      901 ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcatc
      961 gtggccggat cttgcggccc ctcggcttga acgaattgtt agacattatt tgccgactac
     1021 cttggtgatc tcgcct
//
