LOCUS       sequence-71711-e        1120 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1120)
  TITLE     Series-13: Co-transformation vectors for Series-2 transfer vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET StrepII TEV co-transformation cloning vect...
FEATURES             Location/Qualifiers
     source          1..1120
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     125..144
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        125..143
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    148..171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             224..246
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             263..286
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             293..313
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(438..456)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      452..499
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      121 aaattaatac gactcactat agggagagaa ttgtgagcgg ataacaattc ccctgtagat
      181 taattaagcg gccgccctgc aggactcgag ttctagaaat aattttgttt aactttaaga
      241 aggagatata catatggcta gctggagcca cccgcagttc gaaaaaggcg ccgaaaacct
      301 gtacttccaa tccaatattg gaagtggata acggatccgc gatcgcggcg cgccacctgg
      361 tggccggccg gtaccacgcg tgcgcgctga tccggctgct aacaaagccc gaaaggaagc
      421 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      481 ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg tcacactgct
      541 tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg
      601 ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg gcccctcggc ttgaacgaat
      661 tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtaat ggacaaattc
      721 ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct
      781 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
      841 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
      901 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
      961 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
     1021 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
     1081 gatcgtggct ggctcgaaga tacctgcaag aatgtcattg
//
