LOCUS       sequence-71712-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG tagged NmCas9 with no NLS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, 
            Thomson JA
  TITLE     Efficient genome engineering in human pluripotent stem cells using 
            Cas9 from Neisseria meningitidis.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 12.
  PUBMED    23940360
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Aug 12."
COMMENT     Sequence Label: pSimpleII-NmCas9-FLAG
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcgatatcgg catcgcatcc gtcggctggg cgatggtaga aattgacgaa gaagaaaacc
       61 ccatccgcct gattgatttg ggcgtgcgcg tatttgagcg tgccgaagta ccgaaaacag
      121 gcgactccct tgccatggca aggcgtttgg cgcgcagtgt tcgccgcctg acccgccgtc
      181 gcgcccaccg cctgcttcgg acccgccgcc tattgaaacg cgaaggcgta ttacaagccg
      241 ccaattttga cgaaaacggc ttgattaaat ccttaccgaa tacaccatgg caacttcgcg
      301 cagccgcatt agaccgcaaa ctgacgcctt tagagtggtc ggcagtcttg ttgcatttaa
      361 tcaaacatcg cggctattta tcgcaacgga aaaacgaggg cgaaactgcc gataaggagc
      421 ttggcgcttt gcttaaaggc gtagccggca atgcccatgc cttacagaca ggcgatttcc
      481 gcacaccggc cgaattggct ttaaataaat ttgagaaaga aagcggccat atccgcaatc
      541 agcgcagcga ttattcgcat acgttcagcc gcaaagattt acaggcggag ctgattttgc
      601 tgtttgaaaa acaaaaagaa tttggcaatc cgcatgtttc aggcggcctt aaagaaggta
      661 ttgaaaccct actgatgacg caacgccctg ccctgtccgg cgatgccgtt caaaaaatgt
      721 tggggcattg caccttcgaa ccggcagagc cgaaagccgc taaaaacacc tacacagccg
      781 aacgtttcat ctggctgacc aagctgaaca acctgcgtat tttagagcaa ggcagcgagc
      841 ggccattgac cgataccgaa cgcgccacgc ttatggacga gccatacaga aaatc
//
