LOCUS       sequence-71731-e        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  TITLE     Series-13: Co-transformation vectors for Series-2 transfer vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 Mocr TEV co-transformation cloning ve...
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     125..144
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        125..143
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    148..171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             224..246
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             266..283
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             653..673
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(798..816)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      812..859
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      121 aaattaatac gactcactat agggagagaa ttgtgagcgg ataacaattc ccctgtagat
      181 taattaagcg gccgccctgc aggactcgag ttctagaaat aattttgttt aactttaaga
      241 aggagatata catatgaaat cttctcacca tcaccatcac catggttctt ctatgagcaa
      301 catgacatac aataatgttt tcgaccacgc gtacgaaatg cttaaggaga acatccgtta
      361 tgatgacata cgggatacag atgaccttca cgacgcaatt catatggccg ccgataatgc
      421 cgtccctcac tattatgccg atattcggtc agttatggca tctgaaggta ttgatctgga
      481 atttgaggat agtggcctca tgccagatac gaaggacgac atcagaattc tgcaagcgcg
      541 gatctacgaa caacttacca tcgatttatg ggaagatgcg gaagatctcc tgaatgaata
      601 cctggaagaa gtggaggagt atgaggaaga tgaagagggg acggggatcg aggaaaacct
      661 gtacttccaa tccaatattg gaagtggata acggatccgc gatcgcggcg cgccacctgg
      721 tggccggccg gtaccacgcg tgcgcgctga tccggctgct aacaaagccc gaaaggaagc
      781 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
      841 ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg tcacactgct
      901 tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg
      961 ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg gcccctcggc ttgaacgaat
     1021 tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgta
//
